Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed (ab6947)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
- Conjugation: Cy5.5 ®. Ex: 678nm, Em: 703nm
- Host species: Goat
- Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopymore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native Mouse IgG (purified).
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標識
Cy5.5 ®. Ex: 678nm, Em: 703nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
特記事項(標識)
Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 3.7 moles Cy5.5 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
特記事項
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6947の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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Flow Cyt |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Immunomicroscopy |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
Flow Cyt
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab6947 は 10 報の論文で使用されています。
- Kim SJ et al. Firing of Replication Origins Is Disturbed by a CDK4/6 Inhibitor in a pRb-Independent Manner. Int J Mol Sci 24:N/A (2023). PubMed: 37445805
- Afanasyeva EA et al. Kalirin-RAC controls nucleokinetic migration in ADRN-type neuroblastoma. Life Sci Alliance 4:N/A (2021). PubMed: 33658318
- Bauwens S et al. The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin. Life (Basel) 11:N/A (2021). PubMed: 33804994
- Ben Yamin B et al. DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability. EMBO J 40:e104543 (2021). PubMed: 34533226
- Mendez-Bermudez A et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
- Holloway PM et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
- Wilhelm T et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
- Técher H et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
- Letessier A et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320