Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)

製品の概要

  • 製品名
    Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed
  • ターゲット生物種
    Mouse
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details
  • 標識
    Cy3 ®. Ex: 552nm, Em: 565nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. PubMed: 24270184
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/2000 - 1/4000.

画像

  • ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.

参考文献

This product has been referenced in:
  • Li Y  et al. Cytotoxic Indole Alkaloid 3a-Acetonyltabersonine Induces Glioblastoma Apoptosis via Inhibition of DNA Damage Repair. Toxins (Basel) 9:N/A (2017). Read more (PubMed: 28452946) »
  • Dong H  et al. Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period. Evid Based Complement Alternat Med 2017:3647080 (2017). Read more (PubMed: 28588637) »

See all 15 Publications for this product

レビューと Q&A

Application
Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. The original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. Our results support this claim and the brightness seen in the red channel during image acquisition is considerable. Required exposure times/intensity settings for imaging are, consequently, very low compared to similar fluorophores.
The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (594 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.
Username

Dr. Sam Nowitzki

Verified customer

投稿 Jul 09 2015

Thank you for your reply.
I'm pleased to hear you would like to participate in the testing discount scheme with the Goat anti-Mouse IgG H&L (Cy3 ®) secondary antibody (ab97035). The discount code is as follows:
Discount code: xxxxxxxx
Exp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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