Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033)

製品の概要

  • 製品名Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed
  • ターゲット生物種Mouse
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, ELISAmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/200000. (Chemiluminescent).
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (Primary).

Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033) 使用論文

ab97033 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Used with Timp2 primary antibody ab1828
PAGE: 12%, reducing-denaturing
Blocking with 10% milk overnight
Secondary dilution 1:2000
Detection: ABC system
Band at around 24 kDa
Username

Mrs. Henda Nabli

Verified customer

投稿 Oct 18 2013

Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"