Goat Anti-Mouse IgG H&L (Biotin) (ab6788)
Key features and details
- Goat Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
mouse IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
The conjugate is expected to have between 5 and 10 moles of biotin per mole of IgG.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6788の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/1000 - 1/5000.
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ICC |
Use at an assay dependent dilution. PubMed: 17980964
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
1/350000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Immunomicroscopy |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/20000.
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特記事項 |
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ICC/IF
1/1000 - 1/5000. |
ICC
Use at an assay dependent dilution. PubMed: 17980964 |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/350000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
1/2000 - 1/20000. |
画像
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IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded human normal colon*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was then incubated with ab11080, 1/1,000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10,000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10 min at room temperature. The section was then counterstained with hematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ELISA - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)
ELISA results of purified ab6788 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (79)
ab6788 は 79 報の論文で使用されています。
- Yu XJ et al. BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity. Mol Med 29:30 (2023). PubMed: 36858954
- Elseweidy MM et al. Vitamin D alleviates cognitive dysfunction and brain damage induced by copper sulfate intake in experimental rats: focus on its combination with donepezil. Naunyn Schmiedebergs Arch Pharmacol 396:1931-1942 (2023). PubMed: 36864348
- Sim HJ et al. Osteoblastic Wls Ablation Protects Mice from Total Body Irradiation-Induced Impairments in Hematopoiesis and Bone Marrow Microenvironment. Aging Dis 14:919-936 (2023). PubMed: 37191410
- Chen S et al. Immunomagnetic microscopy of tumor tissues using quantum sensors in diamond. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35082154
- Yamamoto K et al. Protocol for generating a mouse model of gastric MALT lymphoma and the identification of MALT lymphoma cell populations by immunostaining. STAR Protoc 3:101155 (2022). PubMed: 35146453