製品の概要

  • 製品名
    Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Frmore details
  • 免疫原

    mouse IgG whole molecule

  • 標識
    Alkaline Phosphatase

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
  • 特記事項(標識)
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200 - 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/1000 - 1/4000.
Dot blot Use at an assay dependent concentration.
ELISA 1/15000.
IHC-P 1/200 - 1/1000.
IHC-Fr Use at an assay dependent concentration.

画像

  • Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG at 0.1

    Observed band size : 28,55 kDa (why is the actual band size different from the predicted?)

    Incubated with ab6790 for 60 min at room temperature.

    Blocked for 30 min at room temperature.

参考文献

This product has been referenced in:
  • Li X  et al. Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells. Int J Environ Res Public Health 13:N/A (2016). IHC . Read more (PubMed: 26907321) »
  • Anttila K  et al. Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level. J Exp Biol 216:1183-90 (2013). Read more (PubMed: 23487268) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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