Goat Anti-Mouse IgG Fc (HRP) (ab97265)
Key features and details
- Goat Anti-Mouse IgG Fc (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG Fc (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc fagment. Cross reactivity with IgA and IgM is negligible. This antibody may cross react with IgG from other species.
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アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.80
Constituents: 0.2% BSA, 99.75% PBS, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000. (Primary).
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WB |
1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000
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特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. (Primary). |
WB
1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000 |
画像
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IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/200 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab97265 は 45 報の論文で使用されています。
- Williams AJ et al. A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice. Front Mol Biosci 10:1085887 (2023). PubMed: 36936989
- Geng S et al. Balancing WNT signalling in early forebrain development: The role of LRP4 as a modulator of LRP6 function. Front Cell Dev Biol 11:1173688 (2023). PubMed: 37091972
- Yuan M et al. An RBD bispecific antibody effectively neutralizes a SARS-CoV-2 Omicron variant. One Health Adv 1:12 (2023). PubMed: 37521533
- Prakash H et al. Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk. Sci Rep 12:9475 (2022). PubMed: 35676314
- Guerrero S et al. Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis. Biosensors (Basel) 12:N/A (2022). PubMed: 36005006