Goats were immunized with a highly purified mouse IgE hybridoma protein in CFA
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0.01M Tris-0.14M sodium chloride pH 7-7.2
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Immunogen affinity purified
The antiserum was solid phase absorbed to ensure class specificity.
The anti epsilon antibodies were immunoaffinity purified off an immunoabsorbant containing mouse IgE protein different from that used for immunization.