Goat Anti-Mouse IgA alpha chain (HRP) (ab97235)
Key features and details
- Goat Anti-Mouse IgA alpha chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgA alpha chain (HRP)
IgA 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
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アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
免疫原
Other Immunogen Type corresponding to Mouse IgA.
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97235の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000. (primary).
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WB |
1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000
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特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. (primary). |
WB
1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000 |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab97235 は 40 報の論文で使用されています。
- Chen S et al. Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines. Small 19:e2205819 (2023). PubMed: 36564365
- Watanabe Y et al. Identification of Closed Linear Epitopes in S1-RBD and S2-HR1/2 of SARS-CoV-2 Spike Protein Able to Induce Neutralizing Abs. Vaccines (Basel) 11:N/A (2023). PubMed: 36851165
- Stoler-Barak L et al. B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation. Nat Commun 14:1462 (2023). PubMed: 36927854
- Li Y et al. Gut bacteria induce IgA expression in pituitary hormone-secreting cells during aging. iScience 26:107747 (2023). PubMed: 37692284
- Kou Y et al. The HVEM-BTLA Immune Checkpoint Restrains Murine Chronic Cholestatic Liver Injury by Regulating the Gut Microbiota. Front Immunol 13:773341 (2022). PubMed: 35185877