Anti-Human IgM (µ-chain specific) is prepared from
goat anti-human IgM antiserum by immunospecific
purification to remove essentially all goat serum protein,
including immunoglobulins, which do not bind
specifically to the µ-chain of human IgM.
Conjugation of the antibody preparation to Alkaline Phosphatase is accomplished by protein cross-linking with 0.2% glutaraldehyde.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: A minimum titer of 1:7,000 is determined by Direct ELISA. Titer is defined as the dilution of conjugate sufficient to give a change in absorbance of 1.0 at 405 nm after 30 minutes of substrate conversion at 25 °C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
has not yet been referenced specifically in any publications.