バッファー50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
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精製度Immunogen affinity purified
特記事項（精製）From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG.
Specific antibodies were absorpted by incubation with Sepharose-bound human IgM.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human IgM a minimum of 65% protein bound.
特記事項（標識）The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
特記事項F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited.