製品の概要

  • 製品名Goat Anti-Human IgM H&L (FITC)
    IgM 二次抗体 製品一覧
  • ターゲット生物種Human
  • 特異性Antibodies were raised in goat to human IgM
  • アプリケーション適用あり: ICC/IFmore details
  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファー50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound.
  • 特記事項(標識)The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ポリ/モノポリクローナル
  • 軽鎖の種類unknown
  • 特記事項F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
  • 追加情報For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • Goat Anti-Human IgM H&L (FITC) (ab8497) 使用論文

    ab8497 has not yet been referenced specifically in any publications.

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