Goat Anti-Human IgG H&L (HRP) (ab81202)

製品の概要

  • 製品名Goat Anti-Human IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Human
  • 特異性No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • アプリケーション適用あり: WB, ELISAmore details
  • 免疫原

    Human IgG.

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.50
    Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • 特記事項(標識)The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100.
ELISA 1/1000.

Goat Anti-Human IgG H&L (HRP) (ab81202) 使用論文

ab81202 has not yet been referenced specifically in any publications.

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Thank you for contacting us with your questions. Ab81202 was raised against human IgG, and since ab81202 is polyclonal, I expect it to react with both the heavy and light chains. I do not think that this has been specifically tested. Ab81202 has...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"