Goat Anti-Human IgG H&L (HRP) (ab81202)
Key features and details
- Goat Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA
製品の概要
-
製品名
Goat Anti-Human IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Human -
特異性
No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species. -
アプリケーション
適用あり: WB, ELISAmore details -
免疫原
Human IgG.
-
標識
HRP
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. -
特記事項(標識)
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81202の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/100.
|
|
ELISA |
1/1000.
|
特記事項 |
---|
WB
1/100. |
ELISA
1/1000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab81202 は 4 報の論文で使用されています。
- Kazmierczak Z et al. Immune Response to Therapeutic Staphylococcal Bacteriophages in Mammals: Kinetics of Induction, Immunogenic Structural Proteins, Natural and Induced Antibodies. Front Immunol 12:639570 (2021). PubMed: 34194425
- Karaaslan E et al. Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV). PLoS Negl Trop Dis 15:e0009973 (2021). PubMed: 34851958
- Maldonado-Ruiz LP et al. Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony. Front Immunol 10:1996 (2019). PubMed: 31555263
- Londono-Renteria BL et al. Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia. Front Public Health 6:111 (2018). PubMed: 29868532