製品の概要

  • 製品名
    Goat Anti-Human IgG Fc (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Human IgG - Fc (HRP), pre-adsorbed
  • ターゲット生物種
    Human
  • アプリケーション
    適用あり: ICC, IHC-P, ELISA, WBmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).

参考文献

This product has been referenced in:
  • McLean RT  et al. Differential antibody responses to gliadin-derived indigestible peptides in patients with schizophrenia. Transl Psychiatry 7:e1121 (2017). ELISA ; Human . Read more (PubMed: 28485731) »

See 1 Publication for this product

レビューと Q&A

Application
Dot blot
Human IgG1 was used as standard series for Dot blot. 2µl sample (in duplicates) or different dilutions of human IgG1 were blotted on a nitrocellulose membrane (0.45µm, Ultra Cruz). The membrane was incubated for 1h at RT with anti-human IgG Fc antibody (HRP) preadsorbed (ab98624) in TBS-T. The blot was developed using ECL substrate and chemiluminescence detected.


Result: Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) is suitable for Dot blot
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投稿 Sep 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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