製品の概要

  • 製品名Goat Anti-Human IgA H&L (FITC)
    IgA 二次抗体 製品一覧
  • ターゲット生物種Human
  • 特異性Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • アプリケーション適用あり: ICC/IFmore details
  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファー50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • 特記事項(標識)The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ポリ/モノポリクローナル
  • 特記事項F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgA.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
  • 追加情報For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • Goat Anti-Human IgA H&L (FITC) (ab8500) 使用論文

    ab8500 has not yet been referenced specifically in any publications.

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