製品の概要

  • 製品名
    Goat Anti-Human IgA H&L (FITC)
    IgA 二次抗体 製品一覧
  • ターゲット生物種
    Human
  • 特異性
    Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • アプリケーション
    適用あり: ICC/IFmore details
  • 標識
    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • 特記事項(標識)
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ポリ/モノ
    ポリクローナル
  • 特記事項
    F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgA.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
  • 追加情報
    For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • 参考文献

    ab8500 has not yet been referenced specifically in any publications.

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