Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed (ab6120)

製品の概要

  • 製品名Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rat
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • アプリケーション適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 吸着処理血清
    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Rat IgG whole molecule

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
WB
ELISA
Electron Microscopy
IHC
  • 追加情報Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/16000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product.

    Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed (ab6120) 使用論文

    This product has been referenced in:
    • Singh MK  et al. MicroRNA-processing Enzyme Dicer Is Required in Epicardium for Coronary Vasculature Development. J Biol Chem 286:41036-45 (2011). IHC-Fr . Read more (PubMed: 21969379) »
    • Leng X  et al. Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats. Cell Stress Chaperones 15:947-58 (2010). ELISA . Read more (PubMed: 20607472) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab6120.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"