Goat F(ab')2 Anti-Rat IgG - Fc (PE), pre-adsorbed (ab6259)

製品の概要

  • 製品名Goat F(ab')2 Anti-Rat IgG - Fc (PE), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rat
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
  • アプリケーション適用あり: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(c) fragment

  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Immunomicroscopy
Flow Cyt
  • 追加情報Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.

    Suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rat IgG - Fc (PE), pre-adsorbed (ab6259) 使用論文

    ab6259 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab6259.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"