Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517)

製品の概要

  • 製品名Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rat
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • アプリケーション適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 吸着処理血清
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(ab’)2 fragment

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.20
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.16% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517) 使用論文

This product has been referenced in:
  • Motomura E  et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . Read more (PubMed: 25330272) »
  • Wang SH  et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). Read more (PubMed: 23363740) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"