Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab6013)

製品の概要

  • 製品名
    Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rabbit
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • アプリケーション
    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG, whole molecule

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/115000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

参考文献

This product has been referenced in:
  • Chou MH  et al. Effects of hepatocyte CD14 upregulation during cholestasis on endotoxin sensitivity. PLoS One 7:e34903 (2012). WB . Read more (PubMed: 22511970) »
  • Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). Read more (PubMed: 21362625) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to gen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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