Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009)

製品の概要

  • 製品名Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • アプリケーション適用あり: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG whole molecule

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of Goat IgG F(ab’)2
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed 画像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1 (basement membrane marker), 1:400
    Secondary antibody: Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009), 1:100
    Nuclei were counterstained with DAPI
    F-actin was stained with rhodamine-phalloidin, 1:100

Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009) 使用論文

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