Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485)

製品の概要

  • 製品名Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • アプリケーション適用あり: IHC-P, ELISA, WB, ICCmore details
  • 吸着処理血清
    Human, Mouse, Rat more details
  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. (Colorimetric: /2,000-1/20,000 dilution; Chemiluminescent: 1/5,000-1/25,000 dilution).
ICC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485) 使用論文

ab98485 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
HEK293 cells engineered to stably overexpress the human Leptin Receptor (hLR) were stimulated with increasing doses of recombinant human Leptin (rhLeptin). The cells were fixed using formaldehyde, permeabilized, quenched, and blocked using proprietary buffers and then incubated overnight at 4°C with a rabbit anti-pSTAT3 (Y705) antibody (abcam, ab76315, 1/500 dilution). Subsequently, the cells were washed and incubated 30 min at room temperature with a HRP-conjugated goat anti-rabbit F(ab')2 (abcam, ab98485). The cells were washed and TMB substrate was added. TMB conversion was stopped and absorbance was read at 450 nm. The cells were washed and stained using JanusGreen. JanusGreen was eluted and absorbance was read at 630 nm.
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投稿 Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"