Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (Biotin), pre-adsorbed (ab6111)

製品の概要

  • 製品名
    Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (Biotin), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rabbit
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • アプリケーション
    適用あり: ICC/IF, WB, Dot blot, ELISA, IHC, Immunomicroscopymore details
  • 吸着処理血清

    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG F(ab')2 fragment

  • 標識
    Biotin

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
Dot blot
ELISA
IHC
Immunomicroscopy
  • 追加情報
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    IHC: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/20000 of the reconstitution concentration is suggested for this product.

    Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 参考文献

    ab6111 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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