Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

製品の概要

  • 製品名Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    IgM 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • アプリケーション適用あり: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details
  • 免疫原

    Mouse IgM mu heavy chain

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
IHC 1/500 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/200 - 1/1000.

Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) 画像

  • Ab5929 was used with the primary antibody ab8226 in WB. See Abreview on ab8226.

Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929) 使用論文

ab5929 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positiv...

Read More

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cr...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"