This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200.
Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed (ab98742) 使用論文
This product has been referenced in:
Flies AS et al. PD-L1 Is Not Constitutively Expressed on Tasmanian Devil Facial Tumor Cells but Is Strongly Upregulated in Response to IFN-? and Can Be Expressed in the Tumor Microenvironment. Front Immunol7:581 (2016).
Read more (PubMed: 28018348) »