Goat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed (ab98754)

製品の概要

  • 製品名Goat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性Antiserum has been cross adsorbed against human and rat immunosorbents.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 吸着処理血清
    Human, Rat more details

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1,000-1/30,000 dilution.
WB 1/1000 - 1/30000. Colorimetric: Use 1/1,000-1/10,000 dilution; Chemiluminescent: Use 1/1,000-1/30,000 dilution.

Goat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed (ab98754) 使用論文

ab98754 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.

I confirmed with the lab and am happy to forward their confirmation that ab98754 will detect both the (Fab) and the (Fab’)2 fragments of mouse IgG.

Please note that ab98754 itself is also a (Fab')2...

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Thank you for this information.

I have noted that this primary/secondary antibody combination has been used and reviewed by another customer. Their Abreview includes a detailed protocol which you may wish to review. This review is at:http://...

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Thank you for contacting us.

Indeed, a mouse-on mouse staining can be quite tricky; however, it is not impossible. The background will depend on the fixation method ( perfusion fixed tissue vs. normally fixed tissue), on the tissue itself ( ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"