Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006)

製品の概要

  • 製品名Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Human
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清
    Cow, Horse, Mouse, Rat more details
  • 免疫原

    Full length native Human IgG (purified).

  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.60
    Preservative: 0.05% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006) 使用論文

This product has been referenced in:
  • Alvarez-Cienfuegos A  et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). Read more (PubMed: 27345490) »
  • Tiwari A  et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). Read more (PubMed: 24510768) »

See all 2 Publications for this product

Product Wall

Since there are more than one antibody attached to each phycoerythrin (PE) molecule, the antibody concentration is irrelevant in this case. It only takes one active antibody for a PE-antibody conjugate to bind to its antigen. Other antibody molecule...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"