This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
1/50 - 1/500.
1/50 - 1/200.
DyLight®-Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - Fc (DyLight® 650), pre-adsorbed(ab98593)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
This product has been referenced in:
Langers I et al. Natural killer and dendritic cells collaborate in the immune response induced by the vaccine against uterine cervical cancer. Eur J Immunol44:3585-95 (2014).
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