製品の概要

  • 製品名Goat F(ab) Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • アプリケーション適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 免疫原

    Rabbit IgG whole molecule

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 特記事項(標識)Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171) 使用論文

This product has been referenced in:
  • West A  et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol 27:979-89 (2013). Read more (PubMed: 23598442) »
  • Bayne RA  et al. Prostaglandin E2 as a regulator of germ cells during ovarian development. J Clin Endocrinol Metab 94:4053-60 (2009). Read more (PubMed: 19602557) »

See all 2 Publications for this product

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"