製品の概要

  • 製品名Goat F(ab) Anti-Mouse IgG H&L (FITC)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • アプリケーション適用あり: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • 免疫原

    Mouse IgG whole molecule

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • 追加情報Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab) Anti-Mouse IgG H&L (FITC) (ab6669) 使用論文

    This product has been referenced in:
    • Mogilenko DA  et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). Read more (PubMed: 22271762) »
    • Mogilenko DA  et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011). Read more (PubMed: 22194611) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Application Flow Cytometry
    A431 cells were corrected using accutase.
    After incubation with an anti-EGFR antibody, these cells were stained with this anti-mouse IgG-FITC antibody for 30 min at RT.
    isotype control (Blue) was mouse IgG.
    anti-EGFR antibody (Red)
    Dilution: 1/1000 (1.0x10^6 cells/mL)
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    投稿 Jul 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"