Goat F(ab) Anti-Mouse IgG H&L (Biotin) (ab6822)

製品の概要

  • 製品名Goat F(ab) Anti-Mouse IgG H&L (Biotin)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • アプリケーション適用あり: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IFmore details
  • 免疫原

    Mouse IgG whole molecule

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 特記事項(標識)Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/200000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.

Goat F(ab) Anti-Mouse IgG H&L (Biotin) (ab6822) 使用論文

ab6822 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

This antibody should not be cross reacting with Rat. If customer is thinking about a substitution of anti rat with this antibody then please advice them, it will be a total waste of time and reagents.

I ho...

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Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"