Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109)

製品の概要

  • 製品名Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • アプリケーション適用あり: WB, Electron Microscopy, ICC/IFmore details
  • 免疫原

    Mouse IgG whole molecule.

  • 標識FITC + Gold 1.4nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 0.1% BSA, 20mM PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Chromatographically purified through gel filtration columns.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項

     

    This product is a revolutionary new immunocytochemical reagent. This unique antibody probe contains a molecular label which contains both the 1.4nm ultra-small gold particle and fluorescein, enabling both fluorescence and electron microscope observation of a sample stained in a single labelling procedure. This probe is smaller than a whole IgG molecule, does not aggregate, and fluorescence quenching due to the goold particle is negligible. Normal methologies may be used successfully with this product. Due to some quenching of fluorescence by the gold particle, slightly higher concentrations of antibody are recommended for incubations. A blocking agent of 5% non-fat dried milk has been found to reduce background in some cases: this should be used before incubation with probe (in standard wash/blocking steps), and additionally, the Fluorescein-ultra-small gold probe should be diluted in a solution also containing 5% non-fat dried milk before it is applied.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33109) 使用論文

ab33109 has not yet been referenced specifically in any publications.

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This is not very clear on the datasheet of ab33109, but this is actually a Fab' fragment conjugated to both ultra small ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"