Goat polyclonal Secondary Antibody to Cow IgG - H&L, pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgG and with light chains common to other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
Human, Mouse, Rat
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/30000. Coating 1/100 - 1/500. Primary 1/1000 - 1/30000
1/200 - 1/2000.
1/200 - 1/2000.
1/1000 - 1/30000. Colorimetric 1/1000 - 1/10000. Chemiluminescece 1/1000 - 1/30000.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"