製品の概要

  • 製品名Goat Anti-Chicken IgY H&L (HRP)
    IgY 二次抗体 製品一覧
  • ターゲット生物種Chicken
  • アプリケーション適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    chicken IgY whole molecule

  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)Horseradish Peroxidase (HRP)
  • ポリ/モノポリクローナル
  • アイソタイプIgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/10000.
Dot blot Use at an assay dependent dilution.
ELISA 1/50000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/20000. PubMed: 16543490

Goat Anti-Chicken IgY H&L (HRP) 画像

  • IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.
  • Ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

Goat Anti-Chicken IgY H&L (HRP) (ab6877) 使用論文

This product has been referenced in:
  • McCabe MJ  et al. Novel application of luciferase assay for the in vitro functional assessment of KAL1 variants in three females with septo-optic dysplasia (SOD). Mol Cell Endocrinol 417:63-72 (2015). WB ; Human . Read more (PubMed: 26375424) »
  • Jensen SA  et al. C-terminal propeptide is required for fibrillin-1 secretion and blocks premature assembly through linkage to domains cbEGF41-43. Proc Natl Acad Sci U S A 111:10155-60 (2014). Read more (PubMed: 24982166) »

See all 13 Publications for this product

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Abreviews
Application Western blot
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投稿 Mar 15 2016

This antibody is a goat IgG anti-chicken IgY. We apologise for any mistakes on the vial label.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"