製品の概要

  • 製品名Goat Anti-Chicken IgY H&L (FITC)
    IgY 二次抗体 製品一覧
  • ターゲット生物種Chicken
  • アプリケーション適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • 免疫原

    chicken IgY whole molecule

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
  • ポリ/モノポリクローナル
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Goat Anti-Chicken IgY H&L (FITC) 画像

  • Ab6873 was used at dilution 1/500 with the primary antibody ab39376 in ICC/IF. See the review on ab39376.
  • Ab6873 was used at dilution 1/500 with the primary antibody ab13991 in IHC-Fr. See the review on ab13991.

Goat Anti-Chicken IgY H&L (FITC) (ab6873) 使用論文

This product has been referenced in:
  • Munera D  et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun 5:3080 (2014). Read more (PubMed: 24445323) »
  • Millet YA  et al. Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria. PLoS Pathog 10:e1004405 (2014). IF ; Human . Read more (PubMed: 25275396) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Antibody was diluted 1/1000 in PBS with 1% goat serum and incubated 1h at RT
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投稿 Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"