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chicken IgY whole molecule
Our Abpromise guarantee covers the use of ab6876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/3000.|
|Dot Blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|WB||1/3000 - 1/30000.|
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.