Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column.
Affinity purified Goat anti-chicken IgY antibodies were conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v).
Binding Capacity is 1.0 mg of Chicken IgY per ml bed volume.