製品の概要
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製品名Anti-GNL3L antibody
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製品の詳細Rabbit polyclonal to GNL3L
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
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免疫原
Synthetic peptide designed within residues: QAAREQERQK RRTIESYCQD VLRRQEEFEH KEEVLQELNM FPQLDDEATR, corresponding to amino acids 72 - 121 of Human GNL3L (NP_061940).
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ポジティブ･コントロール
- Fetal lung lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab94862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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関連性GNL3L is required for normal processing of ribosomal pre-rRNA and cell proliferation
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細胞内局在Nucleus; nucleolus.
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参照データベース
- Entrez Gene: 507679 Cow
- Entrez Gene: 54552 Human
- Entrez Gene: 100034253 Rat
- Omim: 300873 Human
- SwissProt: Q3T0J9 Cow
- SwissProt: Q9NVN8 Human
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別名
- FLJ10613 antibody
- Guanine nucleotide binding protein-like 3 (nucleolar)-like antibody
- novel GTPase antibody
- OTTHUMP00000023374 antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-GNL3L antibody (ab94862)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GNL3L with ab94862 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.
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Western blot - Anti-GNL3L antibody (ab94862)Anti-GNL3L antibody (ab94862) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 66 kDa
Gel concentration 12%
参考文献
This product has been referenced in:
- Thoompumkal IJ et al. GNL3L Is a Nucleo-Cytoplasmic Shuttling Protein: Role in Cell Cycle Regulation. PLoS One 10:e0135845 (2015). IP . Read more (PubMed: 26274615) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"