Synthetic peptide designed within residues: QAAREQERQK RRTIESYCQD VLRRQEEFEH KEEVLQELNM FPQLDDEATR, corresponding to amino acids 72 - 121 of Human GNL3L (NP_061940).
Our Abpromise guarantee covers the use of ab94862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GNL3L with ab94862 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"