Customer reviews and FAQs

製品の概要

  • 製品名Anti-GNL3L antibody
    GNL3L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GNL3L
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide designed within residues: QAAREQERQK RRTIESYCQD VLRRQEEFEH KEEVLQELNM FPQLDDEATR, corresponding to amino acids 72 - 121 of Human GNL3L (NP_061940).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Fetal lung lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG

アプリケーション

Our Abpromise guarantee covers the use of ab94862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-GNL3L antibody 画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GNL3L antibody (ab94862)
    Immunocytochemistry/ Immunofluorescence - Anti-GNL3L antibody (ab94862)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GNL3L with ab94862 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Western blot - GNL3L antibody (ab94862)
    Western blot - GNL3L antibody (ab94862)
    Anti-GNL3L antibody (ab94862) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Gel concentration 12%

Anti-GNL3L antibody (ab94862) 使用論文

ab94862 has not yet been referenced specifically in any publications.

ab94862 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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1 Abreview or Q&A

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Western blot abreview for Anti-GNL3L antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human HEK 293T)
Loading amount 20 µg
Specification human HEK 293T
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Username

Dr. Pankaj Gupta

Verified customer

投稿 Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"