製品の概要

  • 製品名
    Anti-GNB1L antibody
  • 製品の詳細
    Rabbit polyclonal to GNB1L
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Zebrafish
  • 免疫原

    A region within synthetic peptide: RVFHWRTMQP LAVLAFHSAA VQCVAFTADG LLAAGSKDQR ISLWSLYPRA, corresponding to amino acids 278-327 of Human GNB1L

  • ポジティブ・コントロール
    • Jurkat cell lysate, human kidney and skin tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:12500

ターゲット情報

  • 組織特異性
    Ubiquitous. Highly expressed in heart, liver, skeletal muscle, kidney, spleen, thymus and pancreas. Detected at low levels in lung, placenta and brain.
  • 配列類似性
    Contains 6 WD repeats.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGCRK3 antibody
    • ESTM55 antibody
    • fb98e06 antibody
    • fj09d12 antibody
    • FKSG1 antibody
    • G protein subunit beta-like protein 1 antibody
    • G-protein beta subunit-like protein antibody
    • GNB1L antibody
    • GNB1L_HUMAN antibody
    • guanine nucleotide binding protein (G protein), beta polypeptide 1-like antibody
    • guanine nucleotide binding protein beta-subunit-like polypeptide antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 1 antibody
    • GY2 antibody
    • KIAA1645 antibody
    • WD repeat-containing protein 14 antibody
    • WD40 repeat-containing protein deleted in VCFS antibody
    • WDR14 antibody
    • WDVCF antibody
    • wu:fb98e06 antibody
    • wu:fj09d12 antibody
    • zgc:55774 antibody
    see all

画像

  • Anti-GNB1L antibody (ab50998) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab50998 at 4ug/ml staining human GNB1L in epithelial cells of renal tubule in human kidney tissue by Immunohistochemistry, Paraffin embedded tissue.ab50998 at 4ug/ml staining human GNB1L in epithelial cells of renal tubule in human kidney tissue by Immunohistochemistry, Paraffin embedded tissue.
  • ab50998 at 4ug/ml staining human GNB1L in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.ab50998 at 4ug/ml staining human GNB1L in squamous epithelial cells of human skin by Immunohistochemistry, Paraffin embedded tissue.
  • All lanes : Anti-GNB1L antibody (ab50998) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa

参考文献

ab50998 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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