製品の概要

  • 製品名Anti-GNAT3 antibody
    GNAT3 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GNAT3
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-KNQFLDLNLKKEDKE

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 304-318 of Human GNAT3 (NP_001095856.1)

  • ポジティブ・コントロール
    • Human Small Intestine

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
  • 組織特異性Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa.
  • 配列類似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • 翻訳後修飾Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane.
  • 細胞内局在Cytoplasm. Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GDCA antibody
    • Ggust antibody
    • Gnat 3 antibody
    • GNAT3 antibody
    • GNAT3_HUMAN antibody
    • Gtn antibody
    • Guanine nucleotide binding protein alpha transducing 3 antibody
    • Guanine nucleotide binding protein G(t) subunit alpha 3 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-3 antibody
    • Gustducin alpha 3 antibody
    • Gustducin alpha 3 chain antibody
    • Gustducin alpha-3 chain antibody
    see all

Anti-GNAT3 antibody 画像

  • Immunohistochemistry analysis of formalin-Fixed, paraffin-embedded Human Small Intestine with ab113664 at 3.75 µg/ml.

Anti-GNAT3 antibody (ab113664) 使用論文

ab113664 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113664.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"