製品の概要

  • 製品名Anti-GNAT2 antibody
    GNAT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GNAT2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GNAT2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

ターゲット情報

  • 機能Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • 組織特異性Retinal rod outer segment.
  • 関連疾患Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • 配列類似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACHM4 antibody
    • Cone type transducin alpha subunit antibody
    • GNAT 2 antibody
    • GNAT C antibody
    • Gnat2 antibody
    • GNAT2_HUMAN antibody
    • GNATC antibody
    • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
    • Guanine nucleotide binding protein G t subunit alpha 2 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
    • Transducin alpha 2 antibody
    • Transducin alpha-2 chain antibody
    • Transducin alpha2 antibody
    • Transducin cone specific alpha polypeptide antibody
    see all

Anti-GNAT2 antibody 画像

  • All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-GNAT2 antibody (ab97501) 使用論文

This product has been referenced in:
  • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . Read more (PubMed: 26216965) »
  • Sato K  et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . Read more (PubMed: 22736942) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Retinal)
Loading amount 10 µg
Specification Retinal
Gel Running Conditions Reduced Denaturing (10% Tris Glycine Gel)
Blocking step 1:1 PBS: Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

投稿 Aug 24 2012

Thank you for calling Abcam earlier today,

Please find the immunogen sequence for ab97501 below:
********

If there is anything else I can help you with.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"