製品の概要

  • 製品名
    Anti-GMP Synthase antibody
    GMP Synthase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GMP Synthase
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 252-301 (CTALLNRALN QEQVIAVHID NGFMRKRESQ SVEEALKKLG IQVKVINAAH) of Human GMP Synthase, NP_003866

  • ポジティブ・コントロール
    • MCF7 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
  • パスウェイ
    Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
  • 関連疾患
    Note=A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
  • 配列類似性
    Contains 1 glutamine amidotransferase type-1 domain.
    Contains 1 GMP-binding domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GATD7 antibody
    • Glutamine amidotransferase antibody
    • GMP synthase [glutamine hydrolyzing] antibody
    • GMP synthase [glutamine-hydrolyzing] antibody
    • GMP synthetase antibody
    • Gmps antibody
    • GUAA_HUMAN antibody
    • Guanine monophosphate synthase antibody
    • Guanine monphosphate synthetase antibody
    • Guanosine 5' monophosphate synthase antibody
    • MLL/GMPS fusion protein antibody
    • Testicular tissue protein Li 82 antibody
    see all

Anti-GMP Synthase antibody 画像

  • Anti-GMP Synthase antibody (ab87249) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa

プロトコール

Anti-GMP Synthase antibody (ab87249) 使用論文

ab87249 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (egg extract (total and chromatin))
Specification
egg extract (total and chromatin)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録