Anti-GM130 抗体 - Drosophila Golgi/Cis-Golgi Marker (ab30637)


  • 製品名
    Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker
    GM130 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker
  • 由来種
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Fruit fly (Drosophila melanogaster) GM130.

    (Peptide available as ab32335.)

  • ポジティブ・コントロール
    • Drosophila S2 Cells


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab30637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).


  • 機能
    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • 配列類似性
    Belongs to the GOLGA2 family.
  • ドメイン
    Extended rod-like protein with coiled-coil domains.
  • 細胞内局在
    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 別名
    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all


  • The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
  • All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml

    Lane 1 : Drosophila melanogaster S2 cell extract
    Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA

    Predicted band size: 95 kDa
    Observed band size: 95 kDa

  • ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
    Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.
  • All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml

    Lane 1 : Schneider L2 whole cell lysate (ab14893)
    Lane 2 : Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 95 kDa
    Observed band size: 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:
  • Gordon DE  et al. VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane. PLoS Genet 13:e1006698 (2017). Read more (PubMed: 28403141) »
  • Kamalesh K  et al. Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis. J Cell Sci 130:2119-2133 (2017). WB, IHC-P ; Drosophila melanogaster . Read more (PubMed: 28507272) »

See all 38 Publications for this product

レビューと Q&A

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
total larvae, 4 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Evgenia Salta

Verified customer

投稿 Oct 27 2014

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fruit fly (Drosophila melanogaster) Cell (Whole Ovary/egg chamber)
Whole Ovary/egg chamber
Yes - 1% Triton-X100 2h 1% BSA

Dr. Irina Catrina

Verified customer

投稿 Jun 06 2014

Thank you for your enquiry regarding ab30637 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

After reading through the detailed protocol you k...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting Abcam.

I would like to thank you for your response. If the product has gone off we will gladly replace it. However the procedure for this is that we have the protocol used and troubleshooting steps that you have perf...

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Thank you for contacting us.

I am sorry to hear of the difficulties that you have been experiencing using this product. Would you mind sending me the details of your protocol including the tissue type you are working with and your sample pre...

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Thanks you very much for sending us more details about the experiment. I am sorry I am not sure why the antibody staining appears spotty. This antibody was used in many publications without problem. So perhaps you have received a bad vial of this p...

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Thank you for contacting us and for sending the images. I believe the problem is not due to antibody so I would suggest the following - The antibody is showing consistent results in cells outside the marked area. It looks similar as publicati...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Fruit fly (Drosophila melanogaster) Cell (eye disc)
eye disc
Yes - 0.1% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Apr 28 2008