Anti-GM130 抗体 - cis-Golgi Marker (ab31561)


  • 製品名
    Anti-GM130 antibody - cis-Golgi Marker
    GM130 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GM130 - cis-Golgi Marker
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from within residues 950 to the C-terminus of Rat GM130.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa; MCF7.



Our Abpromise guarantee covers the use of ab31561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 111 kDa).


  • 機能
    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • 配列類似性
    Belongs to the GOLGA2 family.
  • ドメイン
    Extended rod-like protein with coiled-coil domains.
  • 細胞内局在
    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all

Anti-GM130 antibody - cis-Golgi Marker 画像

  • ICC/IF image of ab31561 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31561, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-GM130 antibody - cis-Golgi Marker (ab31561) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    Exposure time : 4 minutes

Anti-GM130 antibody - cis-Golgi Marker (ab31561) 使用論文

This product has been referenced in:
  • Lalani SR  et al. Recurrent Muscle Weakness with Rhabdomyolysis, Metabolic Crises, and Cardiac Arrhythmia Due to Bi-allelic TANGO2 Mutations. Am J Hum Genet 98:347-57 (2016). Read more (PubMed: 26805781) »
  • Leung WH  et al. PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. J Immunol 194:2930-41 (2015). Read more (PubMed: 25687758) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa cells)
Yes - 0.2% Triton X-100 in PBS
HeLa cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Sep 27 2017

Je recommanderais un desdeux anti-GM130 suivants :

ab103416 (testé en Western Blot et IHC-P)dont l'immunogèneprésente 87% d'homologie avec la protéine du xénope (voir pièce jointe), http://www.abcam.c...

Read More

Merci pour la séquenceGM130 de Xénope.

Vous n'avez pas à indiquer de code sur la commande originale de l'anticorps que vous voulez tester. Vous indiquerez le code promotionnel :
1) lors de l'envoi de vos résul...

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Western blot
Human Cell lysate - whole cell (HeLa HEK293 NIH-3T3 PC12)
Loading amount
30 µg
HeLa HEK293 NIH-3T3 PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

投稿 Oct 28 2008