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Our Abpromise guarantee covers the use of ab84259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF. Can be paired for Sandwich ELISA with ab9667. Can be used as detection antibody when paired with recommended pair.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.
The detection limit for recombinant human GM-CSF is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.
ab84259 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"