Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
pH: 7.20 Constituent: 99% PBS
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Immunogen affinity purified
ab114987 was purified by affinity chromatography employing an immobilized Rat GM-CSF matrix.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.09 - 0.14 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Rat GM-CSF (0.5ng/ml).
Use a concentration of 0.5 - 2 µg/ml. ab114987, in conjunction with a Biotinylated Anti-Rat GM-CSF as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat GM-CSF.
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.