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製品の概要

  • 製品名
    Anti-Glypican 3 antibody [SP86]
    Glypican 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP86] to Glypican 3
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from C terminal of Human Glypican 3.

  • ポジティブ･コントロール
    • Human liver cancer tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval (boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).

ターゲット情報

  • 機能
    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
  • 組織特異性
    Highly expressed in lung, liver and kidney.
  • 関連疾患
    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
  • 配列類似性
    Belongs to the glypican family.
  • 細胞内局在
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
    • 別名
      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all

    Anti-Glypican 3 antibody [SP86] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)

      Immunohistochemical staining of human liver hepatocellular carcinoma with ab95363.

    • Flow Cytometry - Anti-Glypican 3 antibody [SP86] - C-terminal (ab95363)
      Flow Cytometry - Anti-Glypican 3 antibody [SP86] - C-terminal (ab95363)

      Flow cytometric analysis of rabbit anti-Glypican 3 (SP86) antibody ab98363 (1/100) in HEPG2 cellls (green) compared to negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glypican 3 antibody [SP86] (ab95363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glypican 3 antibody [SP86] (ab95363)
      ab95363, at 1/100 dilution, staining Glypican 3 in formalin-fixed, paraffin-embedded Human liver cancer tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [SP86] - C-terminal (ab95363)
      Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [SP86] - C-terminal (ab95363)

      ICC/IF image of ab95363 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Glypican 3 antibody [SP86] (ab95363) 使用論文

    ab95363 has not yet been referenced specifically in any publications.

    ab95363 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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