The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 66 kDa.
Use a concentration of 10 µg/ml.
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Highly expressed in lung, liver and kidney.
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
ab81263, at 10ug/ml, staining human hepatocellular carcinoma tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary
antibody, HRP conjugated mouse antisheep used at 1:20 dilution. Sections heated for 10 minutes with 10 mM sodium citrate.