製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Glypican 3
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Glypican 3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Kidney Tissue; Human Liver Tissue; HepG2 Whole Cell; HEK293 Whole Cell.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.

ターゲット情報

  • 機能
    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
  • 組織特異性
    Highly expressed in lung, liver and kidney.
  • 関連疾患
    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
  • 配列類似性
    Belongs to the glypican family.
  • 細胞内局在
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DGSX antibody
    • Glypican proteoglycan 3 antibody
    • Glypican-3 [Precursor] antibody
    • Gpc3 antibody
    • GPC3_HUMAN antibody
    • GTR2 2 antibody
    • GTR2-2 antibody
    • Heparan sulphate proteoglycan antibody
    • Intestinal protein OCI 5 antibody
    • Intestinal protein OCI-5 antibody
    • MXR7 antibody
    • OCI 5 antibody
    • OCI-5 antibody
    • OCI5 antibody
    • SDYS antibody
    • Secreted glypican-3 antibody
    • SGB antibody
    • SGBS antibody
    • SGBS1 antibody
    see all

画像

  • All lanes : Anti-Glypican 3 antibody (ab101478) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab101478 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab101478 has not yet been referenced specifically in any publications.

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