Anti-Glypican 1/ GPC1 抗体 (ab73979)

製品の概要

  • 製品名Anti-Glypican 1/ GPC1 antibody
    Glypican 1/ GPC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glypican 1/ GPC1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the N-terminal domain of human Glypican/GPC1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 62 kDa.
IHC-Fr 1/50 - 1/100.

ターゲット情報

Anti-Glypican 1/ GPC1 antibody 画像

  • ICC/IF image of ab73979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glypican 1/ GPC1 antibody (ab73979) 使用論文

ab73979 has not yet been referenced specifically in any publications.

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Thank you for bringing this to our attention. We have not had other complaints about the antibody, and we ordinarily try to offer suggestions to improve the result, but I assume the protocol you have is correct and that you are not interested in contin...

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The immunogen show 80% sequence homology with mouse so it is more likely that the antibody would cross react.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more adv...

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I am sorry to hearthat the customerhas problemswith this antibody. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. We will replace the antibody ...

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I am sorry to let you know that the laboratory has no image to provide, as the antibody has been tested in another lab on Western blot.It has only been tested on Glypican-1 transfected 293T cells lysates as far as...

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I have requested an image from the laboratorzy, however I have not yet received it. I will forward this to you, as soon as we have it. I can however reassure the customer, that we do guarantee this antibody to give g...

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Thank you for your inquiry.

The product ab73979 does not require an MSDS since it doesn't have any preservatives in it. If you need documentation stating this, please let me know.

I hope this informaiton helps. Please contact us wit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"