FITC Anti-Glycophorin A + B 抗体 [HIR2] (ab52452)
Key features and details
- FITC Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-Glycophorin A + B antibody [HIR2]
Glycophorin A + B 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [HIR2] to Glycophorin A + B -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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ポジティブ・コントロール
- Flow Cyt: A subset of erythrocytes in human peripheral blood.
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特記事項
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE. -
一次抗体 備考
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
ポリ/モノ
モノクローナル -
クローン名
HIR2 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
ターゲット情報
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関連性
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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別名
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab52452 は論文での使用が確認できていません。