Anti-Glycophorin A + B 抗体 [HIR2] (FITC) (ab52452)

製品の概要

  • 製品名Anti-Glycophorin A + B antibody [HIR2] (FITC)
    Glycophorin A + B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIR2] to Glycophorin A + B (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • 特記事項

    ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography.
  • 一次抗体 備考ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • ポリ/モノモノクローナル
  • クローン名HIR2
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 20µl for 106 cells.

Use 20µl for 106 cells, or for 100 µl of whole blood.





ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • 細胞内局在Type I membrane protein.
  • 参照データベース
  • 別名
    • CD235a antibody
    • CD235b antibody
    • Erythroid lineage specific membrane sialoglycoprotein antibody
    • Glycophorin Erik antibody
    • Glycophorin HeP2 antibody
    • Glycophorin MiI antibody
    • Glycophorin MiIII antibody
    • Glycophorin MiV antibody
    • Glycophorin MiVI antibody
    • Glycophorin MiX antibody
    • Glycophorin SAT antibody
    • Glycophorin Sta type C antibody
    • GPA antibody
    • GPB antibody
    • GPB.NY antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPB antibody
    • GYPHe.NY antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiVI antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • Mi.V glycoprotein (24 AA) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS 2 antibody
    • PAS 3 antibody
    • PAS2 antibody
    • PAS3 antibody
    • Sialoglycoprotein alpha antibody
    • Sialoglycoprotein delta antibody
    • SS active sialoglycoprotein antibody
    • SS antibody
    see all

Anti-Glycophorin A + B antibody [HIR2] (FITC) (ab52452) 使用論文

ab52452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"