Anti-Glycophorin A + B 抗体 [HIR2] (Biotin) (ab93548)

製品の概要

  • 製品名
    Anti-Glycophorin A + B antibody [HIR2] (Biotin)
    Glycophorin A + B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIR2] to Glycophorin A + B (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • ポジティブ・コントロール
    • Human peripheral blood leukocytes
  • 特記事項
    Mouse monoclonal [HIR2] to Glycophorin A (Biotin) also has a clone number GA-R2.


    Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.
  • ポリ/モノ
    モノクローナル
  • クローン名
    HIR2
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.125µg for 105 cells.

Use 0.125µg for 105 to 108 cells in a final volume of 100 µl.





ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • 細胞内局在
    Type I membrane protein.
  • 参照データベース
  • 別名
    • CD235a antibody
    • CD235b antibody
    • Erythroid lineage specific membrane sialoglycoprotein antibody
    • Glycophorin Erik antibody
    • Glycophorin HeP2 antibody
    • Glycophorin MiI antibody
    • Glycophorin MiIII antibody
    • Glycophorin MiV antibody
    • Glycophorin MiVI antibody
    • Glycophorin MiX antibody
    • Glycophorin SAT antibody
    • Glycophorin Sta type C antibody
    • GPA antibody
    • GPB antibody
    • GPB.NY antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPB antibody
    • GYPHe.NY antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiVI antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • Mi.V glycoprotein (24 AA) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS 2 antibody
    • PAS 3 antibody
    • PAS2 antibody
    • PAS3 antibody
    • Sialoglycoprotein alpha antibody
    • Sialoglycoprotein delta antibody
    • SS active sialoglycoprotein antibody
    • SS antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Chen G  et al. [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria] Zhonghua Xue Ye Xue Za Zhi 18:190-2 (1997). Read more (PubMed: 15622765) »

See 1 Publication for this product

レビューと Q&A

Thank you for contacting us with your questions, and for your patience while I have been in touch with the lab for more information about ab93548.

The lab has informed me that there are approximately 3-7 molecules of biotin per antibody.The c...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Permeabilization
No
Username

Dr. Elodie Segura

Verified customer

投稿 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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